iPS Core
About the Joslin iPS Core
The Joslin iPS Core Facility was created to accelerate research in the stem cell field by facilitating the derivation and distribution of human induced pluripotent stem cell (hiPSC) lines and the generation of variety type of iPSC derived cells, tissues, and organoids. Both healthy and disease-specific hiPSC lines and their derivatives provide us with an opportunity to study the mechanisms of disease and ultimately to develop new treatments.
The iPS Core has three missions to support research:
- Provide expertise to differentiate Human Pluripotent Stem Cells (hPSC), including both human embryonic stem cells (hESC) and human induced pluripotent Stem cells (hiPSC), into a variety type of human cells and tissues such as 𝛽-like cells, islet-like clusters, and vascular smooth muscle cells.
- Offer design and genetic modification of hPSC by CRISPR/Cas9 mediated gene editing to create clonal iPSC populations carrying the genetic alterations.
- Collect, expand, cryopreserve hiPSC lines derived from PBMCs or fibroblasts from individuals with or without type 1 diabetes, type 2 diabetes, metabolic dysfunction, etc., and bank samples in a repository for distribution to research communities.
Furthermore, the core will work with PIs to establish protocols and workflow to produce iPSC derived adipocytes, hepatocytes, myoblasts, skeletal muscle cells. We are open to collaborations to develop new cell types, tissues, and organoids tailored to specific research needs.
By making these services available to Joslin and the wider research community, we aim to foster and enable scientists to focus on biological discovery and therapeutic applications in the study of diabetes, metabolic disorders, endocrine diseases, and other medical conditions, using the hPSC derived model systems.
Services
- hPSC differentiation to produce β-like cells or islet-like clusters
- hPSC differentiation to produce to vascular smooth muscle cells
- hPSC differentiation to produce other type of cells upon requests
- hPSC Gene editing by CRISPR/Cas9
- hiPSC banking and distribution
Chargeback
Chargeback varies depending on service type, project, type and number of cells produced per batch by hPSC differentiation. Please contact our Core Manager, Ping Jiang, MD, at Ping.Jiang@joslin.harvard.edu
Requesting Services
To request services, please contact our Core Manager, Ping Jiang, MD, at Ping.Jiang@joslin.harvard.edu.
Contact Us
For information or question about services and collaboration opportunities, please email a member of our team:
- Dr. Rohit Kulkarni, Core Director, Rohit.Kulkarni@joslin.harvard.edu
- Dr. Ping Jiang, Core Manager, Ping.Jiang@joslin.harvard.edu
Core Leadership & Scientific Oversight
